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In the case of closely related proteins or isozymes, a variation of this approach should be applied to create an affinity gradient, where the concentration of ligand is gradually increasing in the mobile phase
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FIGURE 1 2.1.2 Iron cycle Fenton reaction, the oxidation of Fe 2+ and H 2 O 2 , catalyzes the production of ROS and is the beginning of the non-enzymatic reaction of lipid peroxidation (Hendren et al., 2020)
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