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2.3 Determination of biochemical and inflammatory factors Enzyme-linked immunosorbent assay (ELISA) kits (Nanjing Jiancheng Institute of Biological Engineering, Nanjing, China) were used to measure various biomarkers, including blood lipid contents—total cholesterol (TC), triglycerides (TG), high-density lipoprotein cholesterol (HDL-C), and low-density lipoprotein cholesterol (LDL-C)—as well as blood glucose, lipopolysaccharide (LPS), tumour necrosis factor-alpha (TNF-α), interleukin-1β (IL-1β), IL-10, and IL-6
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The first studies on heteromers of the D 4 R polymorphic variants, based on BRET and co-immunoprecipitation techniques in transfected mammalian cells, suggested that the D 4.7 R establishes weaker intermolecular and functional interactions with the D 2 R (both isoforms, D 2L R and D 2S R) than the D 4.4 R (52, 56, 57)
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