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谷胱甘肽还原酶检测试剂盒(DTNB 法)(Glutathione Reductase Assay Kit with DTNB)是一种简单易行的通过比色法来检测细胞、组织或 其它样品中谷胱甘肽还原酶(Glutathione reductase,GR)活性的试剂盒。谷胱甘肽还原酶在许多组织中都有分布,可以维持细胞内充足的还 原型谷胱甘肽(GSH)水平。GSH 可以清除自由基和一些有机过氧化物,或作为谷胱甘肽氧化酶(Glutathione peroxidase)的底物来清除一些 过氧化物。 谷胱甘肽还原酶可以还原氧化型谷胱甘肽(GSSG)生成还原型谷胱甘肽(GSH),而 GSH 可以和生色底物 DTNB 反应产生黄色的 TNB 和 GSSG,并可以通过测定 A412来检测 TNB 的生成量。适当设置应体系,前后两个反应合并起来后,GSSG/GSH 过量而 GR 相对不足时, 该反应体系中谷胱甘肽还原酶就成为整个反应体系的限速因素,此时黄色的 TNB 生成量和谷胱甘肽还原酶的活性呈线性正相关。从而通 过测定 A412就可以计算出谷胱甘肽还原酶的活性水平。本
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