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Three 10-min washes with wash solution (0.5% (w/v) skim milk powder, 0.1% Tween20, 1x PBS) were conducted, shaking, at room temperature, followed by a 2 h incubation with a secondary antibody (Rabbit IgG HRP Linked F (ab′)2, GENA9340

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They progressively accumulate a variety of post-translational modifications, 18 many of which are associated with aging and not specifically, or preferentially, with cataractogenesis