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The peptide net charge calculator at a given pH is based on the formula below: Z = ∑ i N i · (10 pK ai / (10 pH + 10 pK ai )) − ∑ j N j · (10 pH / (10 pH + 10 pK aj )) Z : Net charge of the peptide sequence N i : Number of arginine, lysine, and histidine residues and the N-terminus pK ai , pK a : values of the N-terminus and the arginine, lysine, and histidine residues N j : Number of of aspartic acid, glutamic acid, cysteine, and tyrosine residues and the C-terminus pK aj , pK a : values of the C-terminus and the aspartic acid, glutamic acid, cysteine and tyrosine residues pH : pH value The pKa values for cysteine (pKa = 8.33), aspartic acid (pKa = 3.86), glutamic acid (pKa = 4.25), histidine (pKa = 6.0), lysine (pKa = 10.53), arginine (pKa = 12.48), tyrosine (pKa = 10.07), the N-terminal (pKa = 9.69) and C-terminal (pKa = 2.34) are based on the Principles of Biochemistry, Lehninger (1982)