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After 4–5 days in culture, the small pieces of tracheal tissue were removed and single cells were trypsinized and re-plated on 10-mm diameter circular, glass cover slips that were pre-coated with 1 mg/ml poly-D-lysine (Sigma)
Beriberi management
Variant:20mg
It is important for older adults to regularly monitor their B12 levels and consider supplements or injections to manage deficiency
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