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While the combination varies by cell type, the following alterations are required in all cases: TERT activation, loss of p53 pathway function, loss of pRb pathway function, activation of the Ras or myc proto-oncogenes, and aberration of the Protein phosphatase 2 (PP2A)

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Experimental evidence supports the importance of the MI/DCI ratio in ovarian physiology, as different formulations induce distinct changes in granulosa and theca cell architecture, with the physiological 40:1 ratio preserving follicular structure compared to DCI-enriched conditions ( Figure 3 )