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For proteomics in particular, the future reliability of clinical and biomarker studies will depend first on our ability to achieve: • good protein and peptide recovery • representative coverage of the proteome • reproducibility across laboratories • standardized workflows suitable for population-level comparisons PTMs, analytical sensitivity and downstream data interpretation introduce additional challenges, but everything starts with accurate and reproducible sample preparation anyway

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Interestingly, we found that the TGFβ levels were substantially elevated in the conditioned media from NP and L929 cocultures (Supplementary Fig