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The cell lysates were filtered through a 0.45 µm syringe filter and purified by affinity chromatography using a 5 mL HisTrap HP column (GE Healthcare Life Sciences) with wash buffer (20 mM Tris–HCl, 500 mM NaCl, pH 7.4) and a gradient of 4-100% elution buffer (20 mM Tris–HCl, 500 mM NaCl, 250 mM imidazole, pH 7.4) over 10 CV

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