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Perls’ DAB staining and non-hem iron measurements 3,3’-Diaminobenzidine (DAB)-enhanced Perls’ staining was used to detect iron accumulation in paraffin embedded lung sections according to the manufacture’s instructions

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To investigate whether direct activation of 5-HT 1A receptors attenuates ASD-like behaviors and hyper-glutamatergic function, prenatal VPA mice were administered TAND (0.1 mg/kg, i.p .)a 5-HT 1A receptor agonist-and subjected to behavioral tests