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After incubation, the cells were washed twice with DPBS, and the fluorescence signals were analysed using flow cytometry with an excitation wavelength of 488 nm and the emission wavelength of 525 nm on a CytoFLEX flow cytometer (Beckman Coulter, USA)

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476 The upregulation of Ki-67 expression in CD8 + T cells indicates clonal expansion, a hallmark of the immune response during effective immunotherapy