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Subsequently, the neurons were treated with PBS or 200 nM human α-syn PFFs for 7 days, followed by infection with lentiviral vectors expressing three ubiquitin variants (WT, K48R, K63R) with HA tag
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This study is limited by its retrospective nature, by incomplete blinding of both patients and treaters, and by the uniquely refractory patient population and the intenstive behavioral and pharmacological management the patients were receive simultaneously
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