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Membranes were blocked with 5% milk in Tris-buffered saline with Tween-20 (TBST) and then incubated with primary antibodies overnight at 4 °C (all antibodies were 1:1,000 in 5% milk in TBST, while GAPDH primary antibody was diluted 1:5,000 in 5% milk in TBST)
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